A complete list of all our most frequently asked questions relating to HAP1 knockout cell lines. If you want to know how they're generated, how they're validated or how to find out if they're right for you - this is a great place to start.
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Much information about the role of specific genes in fundamental biological processes and the onset and progression of genetic disease has been gleaned by researchers having the ability to selectively alter the genomic composition of individual genes and study the consequences. This approach enables researchers to observe the effects of a specific mutation, SNP or deletion in combination with the added layers of regulation present within the cell, including post-translational modification, epigenetic changes associated with chromatin structure, and transcriptional mechanisms.
Following transfection of CRISPR reagents, cells will need to be single cell diluted to obtain a clonal population. In this blog, we review the method used in-house.
A review of the main 5 tips to choose and use a cell line. We highlight the importance of checking for authenticity, and contamination.